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Fig. 5 | Mobile DNA

Fig. 5

From: KRAB zinc finger protein ZNF676 controls the transcriptional influence of LTR12-related endogenous retrovirus sequences

Fig. 5

ZNF676 regulates several germline genes involved in cilia motility, and its overexpression is able to disrupt ciliogenesis. A Gene Ontology Cellular Component terms (GOstats R package, hypergeometric test, non-adjusted p-value) for which genes within 50 kb of an LTR12C assigned to the maternal cluster were enriched. B Immunofluorescence staining of normal human bronchoepithelial cells (Lonza) overexpressing HA-tagged LacZ (control) or ZNF676, differentiated into ciliated cells (4 weeks at air-liquid interface). Blue: DAPI (nuclei), green: acetylated tubulin (cilia), purple: HA-tagged lentivector-mediated overexpression of protein of interest (left, LacZ, right, ZNF676). C Representative scanning electron microscopy images of the surface of Au/Pd-coated primary human bronchoepithelial cells (Lonza) differentiated into ciliated cells (4 weeks at air-liquid interface). Magnification: 30’000x. Left: lentivector-mediated overexpression of LacZ (control). Right: lentivector-mediated overexpression of ZNF676. D Gene Ontology Cellular Compartment terms enriched among differentially expressed genes in an RNA-seq of ZNF676-overexpressing NHBE cells compared to GFP-overexpressing ones

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