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Table 1 Bacterial strains and relevant properties

From: Chromosomal integration of Tn5253 occurs downstream of a conserved 11-bp sequence of the rbgA gene in Streptococcus pneumoniae and in all the other known hosts of this integrative conjugative element (ICE)

Strain

Relevant propertiesa

Genome Genbank acc. no., [Reference]

 

Streptococcus pneumoniae

 

A66

Avery’s strain, clinical isolate, serotype 3

LN847353.1, draft genome, [41, 42]

HB565

A66 derivative, carrying str-1, SmR

[39, 43]

FR39

HB565 transconjugant derivative, carrying Tn5253, SmR, TcR, CmR

This study

TIGR4

Clinical isolate, serotype 4

NC_003028.3, [44]

FP47

TIGR4 derivative, carrying nov-1, NovR

[29]

FR54

FP47 transconjugant derivative, carrying Tn5253, NovR, TcR, CmR

[29]

SP18-BS74

Clinical isolate, serotype 18 C

NZ_ABAE01000001.1, draft genome, [45]

FR55

SP18-BS74 derivative, carrying str-1, SmR

[28]

FR56

FR55 transconjugant derivative, carrying Tn5253, SmR, TcR, CmR

[28]

 

Other streptococci

 

H36B

S. agalactiae, clinical isolate, serotype Ib

NZ_LN847353.1, [46]

FR67

H36B transconjugant derivative, carrying Tn5253, TcR, CmR

[29]

SF370

S. pyogenes, clinical isolate, serotype M1

AE004092.2, [47]

FR68

H36B transconjugant derivative, carrying Tn5253, TcR, CmR

[29]

V288

S. gordonii Challis, clinical isolate

NC_009785.1, [48, 49]

GP204

V288 derivative, carrying str-204; SmR

[50]

FR43

GP204 transconjugant derivative, carrying Tn5253, SmR, TcR, CmR

[29]

 

Enterococcus faecalis

 

OG1

Clinical isolate, formely named 2SaR

[51]

OG1RF

OG1 derivative, FusR, RifR

NC_017316.1, [52, 53]

OG1SS

OG1 derivative, SpeR, SmR

[15, 54]

FR49

OG1SS transconjugant derivative, carrying Tn5253, SpeR, SmR, TcR, CmR

[28]

JH2

Clinical isolate

[55]

JH2-2

JH2 derivative, FusR, RifR

NZ_KI518257.1, draft genome, [55]

FR50

JH2-2 transconjugant derivative, carrying Tn5253, FusR, RifR, TcR, CmR

[28]

  1. a str-41 and str-204 indicate point mutations conferring resistance to streptomycin, while nov-1 to novobiocin. Sm, streptomycin; Tc, tetracycline,
  2. Cm, chloramphenicol; Nov, novobiocin; Fus, fusidic acid; Rif, rifampicin; Spe, spectinomycin;