Fig. 1
From: melRNA-seq for Expression Analysis of SINE RNAs and Other Medium-Length Non-Coding RNAs
melRNA-seq analysis workflow. Schematic representation of melRNA-seq analysis. The experimental part consists of total RNA preparation, 5′ processing by RppH, adaptor ligation, reverse transcription, PCR amplification and size selection of the PCR products (left). After obtaining the sequences by MiSeq, they are trimmed to remove the adaptor sequence, and annotated by repeatmasker. Repeat-derived reads are mapped onto the reference genome (right). Mapped reads for individual loci are counted to calculate their expression levels