Fig. 2

NGS analysis of transcribed HERVW ENV copies in PBMCs. A specific MSRV ENV PCR assay [15] was applied to random primed cDNA from CIS patients (n=5) and Controls (n=5) and products were sequenced by NGS. For each sample, the relative frequency (%) of reads mapping to individual HERVW ENV copies was calculated relative to the total number of mapped reads. A) The total number of transcribed HERVW ENV copies identified (p=0.018; student’s T test). B) Individual HERVW ENV copies and the mean relative frequency (%) of reads mapping to each copy is represented in pie charts. The most abundantly transcribed copies are indicated. C-E) The median relative frequency (%) of reads mapped to the individual HERV-W ENV copies in CIS patients or controls is indicated