Fig. 7
From: The IS6 family, a clinically important group of insertion sequences including IS26
Summary of analysis of TU formation from the mutant transposon Tn4352B. The authors [106] used an IS26-based transposon, Tn4352B, carrying the aphA1 gene in which the right hand IS fortuitously carried an additional GG dinucleotide at the left internal end of one of the component IS26 copies to generate a GGGGG pentanucleotide at the IS tip. This appears to render the transposon unstable resulting in an excision of a non-replicative circle, called a translocatable element (TU), carrying a single IS copy and the aphA1 gene. The other partner, the parental plasmid from which the TU had been excised, retained one IS copy and the original 8 base pair direct target repeat (framed in red). The sequence of the IS flanks in the TU were not reported. Symbols are the same as those used in Fig. 6