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Fig. 6 | Mobile DNA

Fig. 6

From: The IS6 family, a clinically important group of insertion sequences including IS26

Fig. 6

Intramolecular transposition. Symbols are identical to those in Fig. 5. The red dotted lines represent the DNA segment between the resident IS and its intramolecular target shown as a white arrow and marked “0”. In addition, a and b represent two markers on this DNA segment. The 3′-OH groups generated by cleavage at both IS ends can either attack the target site on the same strand (cis) (top pathway) or the opposite strand (trans) (bottom pathway). When in cis, DNA between the IS and target site is deleted as a circle containing the markers “a” and “b”, one IS copy flanked by one copy of the original flank, 2, and one copy of the target flank, 0. The other partner also contains a single IS copy with one copy of the original flank, 1, and one copy of the target flank, 0. When the reaction occurs in trans, DNA between IS and target site is instead inverted (“a b” becomes “b a”), bracketed by the original IS and a new copy in an inverted orientation. The target site is also duplicated but in inverted orientation, and one copy of the original flank and one copy of the target flank is associated with each IS copy

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