Fig. 2

RTP-up mutant identification. a. High-throughput RTP (HTP-RTP) assay. All ISS1 mutant and control strains were grown, induced and plated on kanamycin to identify mutants with a putative RTP-up phenotype. Spot size on selective media was quantified relative to the wild-type pLNRK-RIG control (white oval) and visualized as double gradient heatmaps (min, 0; max, 9; baseline, 1). Additional controls included the same strain with pRS01 with an “up” phenotype [21] (pink oval) and a blank well containing only media (black oval). Mutants were ranked into tiers based on the boxplot analysis of retrotransposition frequencies within each plate (Additional File 1: Fig. S5 and Table S4). A box with a solid black outline identifies mutants in the first tier, whereas a dotted outline represents the second tier. b. Results of three independent HTP-RTP assays (RTP 1 – RTP 3) for plate 1 (PL1). Heatmaps for each plate are shown. A contaminated area of the RTP3 plate was excluded from analysis (white area in PL1 RTP 3). Results of the HTP-RTP assays for all plates are in Additional File 1: Fig. S5 and Table S4. c. Boxplot analysis of PL1. RTP levels are reported relative to the wild type. Box and whisker plots outline the quartiles, with the bold line within each box as the median. All boxplots are in Additional File 1: Fig. S5