Skip to main content
Fig. 6 | Mobile DNA

Fig. 6

From: LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites

Fig. 6

Quantitation of piggyBac integrations over a range of concentrations. a) Triplicate libraries were analysed from three cell lines derived from mouse neuronal precursors transfected with piggyBac and cloned by single cell sorting. These DNAs have 1, 5 and 9 integrations each. Triplicate libraries of uninfected DNA did not contain any mappable reads. b) These three DNAs were mixed with each other at ratios of 1:2:4, 4:2:1, 1:5:25 and 25:5:1 and triplicate libraries were constructed. The clonality of each of the integrations is plotted against the percentage of its source DNA present in each mixture. Plots 1–9 are inserts from cell line AltH2B_1 C1 (G2). Plot 10 is the insert from cell line Orig C1 (G8). Plots 11–15 are the inserts of the sample AltH2B_2 C1 (G14)

Back to article page

Mobile DNA

ISSN: 1759-8753