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Fig. 5 | Mobile DNA

Fig. 5

From: LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites

Fig. 5

Quantitation of MuLV integrations over a range of concentrations. a) Triplicate libraries were analysed from two MuLV infected spleen DNA samples, identifying nine clonal integrations in sample #5036 and two clonal integrations in sample #5238. Integration 9 from sample #5036 and integration 1 from sample #5238 both map to the same base pair in the 3′ UTR of Mycn (chr12:12936986) which is a highly selected hotspot for integrations in MuLV infected lymphoma samples. Triplicate libraries of uninfected DNA did not contain any mappable reads. b) These two DNAs were mixed with each other at ratios of 1:49, 1:4, 1:1, 4:1 and 49:1 and triplicate libraries were constructed. The clonality of each of the integrations is plotted against the percentage of its source DNA present in each mixture. Plots 1–8 are inserts 1–8 from sample #5036. Plot 9 is insert 2 from sample #5238. Plot 10 simultaneously represents insert 9 from sample #5036 and insert 1 from sample #5238

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Mobile DNA

ISSN: 1759-8753