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Table 3 Transposition frequencies of two Hsmar1 transposase mutants expressed at optimal and high level

From: Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters

ConstructTransposition frequencyMutant/W.T.
Ip-EE+ W.T.4.73 (±1.02) ×  10−5 
Ip-EE+ F132A9.73 (±4.53) ×  10−421
Ip-EE+ R141L2.42 (±1.68) ×  10− 45
Ip6++ W.T.3.22 (±1.02) ×  10− 7 
Ip6++ F132A5.79 (±2.63) ×  10−5180
Ip6++ R141L3.24 (±1.43) ×  10−41006
  1. The bacterial mating-out assays have been done with the RC5097 strain and the Ip-EE+ or Ip6++ vectors. Transposition frequencies are the average of three independent experiments ± standard error of the mean