Skip to main content

Table 3 Transposition frequencies of two Hsmar1 transposase mutants expressed at optimal and high level

From: Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters

Construct

Transposition frequency

Mutant/W.T.

Ip-EE+ W.T.

4.73 (±1.02) ×  10−5

 

Ip-EE+ F132A

9.73 (±4.53) ×  10−4

21

Ip-EE+ R141L

2.42 (±1.68) ×  10− 4

5

Ip6++ W.T.

3.22 (±1.02) ×  10− 7

 

Ip6++ F132A

5.79 (±2.63) ×  10−5

180

Ip6++ R141L

3.24 (±1.43) ×  10−4

1006

  1. The bacterial mating-out assays have been done with the RC5097 strain and the Ip-EE+ or Ip6++ vectors. Transposition frequencies are the average of three independent experiments ± standard error of the mean
Back to article page

Mobile DNA

ISSN: 1759-8753