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Fig. 7 | Mobile DNA

Fig. 7

From: Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters

Fig. 7

Covalently linking two Hsmar1 monomers in a dimer or mutating Hsmar1 dimer interface affect the transposition rate. a. Representative colonies of each expression vector expressing either Hsmar1 monomer (pRC1868–1871, 1873, 1875, and 1876) or Hsmar1 single chain dimer (pRC1858–1861, 1863, 1865, and 1866). b. Quantification of the number of papillae per colony from single colonies. Average ± standard deviation of six representative colonies from the same biological replicate. c. Different Hsmar1 mutants have been tested in low, optimal and high transposase expression level (Bp1+ (pRC1739 and 1740), Ip1+ (pRC1746 and 1747) and Ip6++ (pRC1752 and 1753), respectively). Representative colonies of each papillation plate is shown. d. Quantification of the number of papillae per colony from single colonies. Average ± standard deviation of six representative colonies from the same biological replicate

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