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Fig. 5 | Mobile DNA

Fig. 5

From: Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters

Fig. 5

Characterization of the papillation assay with the wild-type untagged Hsmar1 transposase and optimal RBS. a. Representative colonies of each vector expressing a wild-type untagged Hsmar1 transposase (pRC1723–1728 and pRC1730–1735). b. Quantification of the number of papillae per colony from single colonies. Average ± standard deviation of six representative colonies from the same biological replicate. c. Plot of the EGFP production relative to Ip6++, determined in Fig. 4c, versus the average number of papillae per colony (as defined in Fig. 5b). As expected from overproduction inhibition (OPI), the promoter strength is inversely related to the level of transposition

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