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Fig. 2 | Mobile DNA

Fig. 2

From: Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters

Fig. 2

SETMAR transposition activity was lost during the same period as Hsmar1 transposase domestication. a. Phylogenetic tree of anthropoid primates which represents the emergence of mutations in the Hsmar1 domain of SETMAR. All the mutations present in the human SETMAR were tested by papillation assay to determine their effects on Hsmar1 transposition. The colour code used for the effect of the mutation on the number of papillae per colony is based on Fig. 2c. b. Representative colonies of pMAL-C2X expressing wild-type (pRC1721) or mutant Hsmar1 transposases (pRC1877–1899). The papillation assays were performed in presence of 0.01% lactose and no IPTG. c. Quantification of the number of papillae per colony from single colonies. Average ± standard deviation of six representative colonies from the same biological replicate

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Mobile DNA

ISSN: 1759-8753