Table 6 Primers used
Gene | qPCR primer | Sequence (5′-3′) | Amplicon length (nt) | PCR protocol |
|---|---|---|---|---|
ICEHo-I_dcm | 463_F | CACGGATCTCCTTGTCAAGAT | ||
463_R | TGTTTCCCACAATAAACTACTTCG | 91 | 1 | |
ICEHo-I_CDS5 | 462_F | AGAAGATTTGTCAAAAACTCCTAAAGA | ||
462_R | ACCACTTTGTGCTTCGGCTAA | 64 | 1 | |
ICEHo-I_CDS14 | 474_F | CCAAATCCTTCAAACCCAACT | ||
474_R | TCTGGTTTAACTTCAGGGGTTG | 62 | 1 | |
ICEHo-I_CDS16 | 476_F | GCAATTGCTTTTGTTGGAAGT | ||
476_R | CTGATCTTGCTCCAGACATAGC | 73 | 1 | |
ICEHo-I_CDS17.1 | 477_F1 | GATTTTGTGCCGTCATCGTA | ||
477_R1 | TTTAAAATGGCAGGATTATCAGG | 67 | 1 | |
ICEHo-I_CDS17.2 | 477_F2 | RAAAATATTTGCAAGAACATAACATTA | ||
477_R2 | ATTTTCTAACCGTTTTTGTCATTT | 165 | 1 | |
ICEHo-II_CDS17 | 17-II_F | CCCAATAAATCCGATAGCATTA | ||
17-II_R | GTTCCCAACACTAACATTCCTC | 84 | 1 | |
circular ICEHo-I | cICE_I-F | GCGGGCGCGTAGAGCAT | ||
cICE_I-R | TATTTGGAATTAACCCCACATTTT | 185 | 2 | |
circular ICEHo-II | cICE_II-F | CAAAATTCAGATTAATTACTAATAAACAAA | ||
cICE_II-R | AGAGCATGAGCAAGAAAAAAAGTA | 290 | 2 | |
hitA | hitA_F | TTGAGGCACAGCAATAGC | ||
hitA_R | AAGGCTTAGGTAAGGAATTGATTAG | 81 | 1 | |
gap | gap_F | GCAGGCTCAATATTTGACTCACT | ||
gap_R | GATGATTCATTGTCGTATCATGC | 95 | 1 |