|
Gene
|
qPCR primer
|
Sequence (5′-3′)
|
Amplicon length (nt)
|
PCR protocol
|
|---|
|
ICEHo-I_dcm
|
463_F
|
CACGGATCTCCTTGTCAAGAT
| | |
| |
463_R
|
TGTTTCCCACAATAAACTACTTCG
|
91
|
1
|
|
ICEHo-I_CDS5
|
462_F
|
AGAAGATTTGTCAAAAACTCCTAAAGA
| | |
| |
462_R
|
ACCACTTTGTGCTTCGGCTAA
|
64
|
1
|
|
ICEHo-I_CDS14
|
474_F
|
CCAAATCCTTCAAACCCAACT
| | |
| |
474_R
|
TCTGGTTTAACTTCAGGGGTTG
|
62
|
1
|
|
ICEHo-I_CDS16
|
476_F
|
GCAATTGCTTTTGTTGGAAGT
| | |
| |
476_R
|
CTGATCTTGCTCCAGACATAGC
|
73
|
1
|
|
ICEHo-I_CDS17.1
|
477_F1
|
GATTTTGTGCCGTCATCGTA
| | |
| |
477_R1
|
TTTAAAATGGCAGGATTATCAGG
|
67
|
1
|
|
ICEHo-I_CDS17.2
|
477_F2
|
RAAAATATTTGCAAGAACATAACATTA
| | |
| |
477_R2
|
ATTTTCTAACCGTTTTTGTCATTT
|
165
|
1
|
|
ICEHo-II_CDS17
|
17-II_F
|
CCCAATAAATCCGATAGCATTA
| | |
| |
17-II_R
|
GTTCCCAACACTAACATTCCTC
|
84
|
1
|
|
circular ICEHo-I
|
cICE_I-F
|
GCGGGCGCGTAGAGCAT
| | |
| |
cICE_I-R
|
TATTTGGAATTAACCCCACATTTT
|
185
|
2
|
|
circular ICEHo-II
|
cICE_II-F
|
CAAAATTCAGATTAATTACTAATAAACAAA
| | |
| |
cICE_II-R
|
AGAGCATGAGCAAGAAAAAAAGTA
|
290
|
2
|
|
hitA
|
hitA_F
|
TTGAGGCACAGCAATAGC
| | |
| |
hitA_R
|
AAGGCTTAGGTAAGGAATTGATTAG
|
81
|
1
|
|
gap
|
gap_F
|
GCAGGCTCAATATTTGACTCACT
| | |
| |
gap_R
|
GATGATTCATTGTCGTATCATGC
|
95
|
1
|
