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Table 3 Integration sites of mobile genetic elements (MGE)

From: Characterisation of mobile genetic elements in Mycoplasma hominis with the description of ICEHo-II, a variant mycoplasma integrative and conjugative element

MGE

Gene / contiga

PG21-homologueb

Gene product

Gene length

(nt)

Insertion site in gene (nt)

DR / IR c

FBG_ISMhom-1_1

dBHBFJMJE_00070rc d

IGR e (dMHO_0640)

AAAATA

FBG_ISMhom-1_2

dBHBFJMJE_00328 d

IGR e (dMHO_2560)

ATATTT

FBG_ISMhom-1_3

dBHBFJMJE_00386 d

IGR e

(dMHO_3070)

TAAATTATTTTGGGT

FBG_ISMhom-1_4

BHBFJMJE_00532rc

MHO_4140rc

put. HAD hydrolase

807

807

AAATAAf

FBG_ISMhom-1_5

BHBFJMJE_00625rc

MHO_5020rc

put. Sporulation transcription regulator WhiA

828

828

AAATAGGCf

SP10291_ISMhom-1

dHPAMDCMO_00271 d

IGR e (dMHO_2210)

TGTGCTTTTT

2539_MHoV1

KLHMLDFE_00358

MHO_3090

hypothetical protein

1134

808

ATTTTTAT / ATTTTTTT

A136_MHoV1

dMNIFKBBE_00264 d

IGR e (dMHO_2260rc)

TTTTTTTT / CTTTTTTT

SP2565_MHoV1

dHHAOGLDO_00056 d

IGR e (dMHO_0530)

ATTTTTATA / ATTTTTCTA

475_MHoV1

dMOHCKDGE_00391 d

IGR e (dMHO_3470)

TTTTTT / CTTTTT

SS10_MHoV1

MFOAEKDO_00548

MHO_4930

hypothetical protein

903

867

CTTTTT

(866–867) g

TO0613_MHoV1

dKN71_002970 d

IGR e (dMHO_4930)

ATTTTTA

FBG_ICEHo-I-1rc

BHBFJMJE_00092

MHO_0820

hypothetical protein

651

394

AAATAATA

(387–394) g

FBG_ICEHo-I-2

BHBFJMJE_00459

MHO_3720

P75 precursor

1950

1950 + 1h

CAAATAAA/AATCTTT i (1323–1950) g

FBG_ICEHo-I-3

BHBFJMJE_00651

MHO_5300

conserved hypothetical protein

432

432 + 2h

AAATAAAAf

(427–432) g

FBG_ICEHo-I-4 vestige

BHBFJMJE_00287

MHO_2250

hypothetical protein

663

44

 

FBG_ICEHo-I-5 vestigerc

dBHBFJMJE_00595 d

IGR e

(dMHO_4730)

 

SP10291_ICEHo-I

dHDENHCDK_00935 d

IGR e (dMHO_4550)

AAATTTTT

TO0613_ICEHo-I-1rc

dKN71_000220 d

IGR e

(dMHO_0170rc)

TTTAAAAT

TO0613_ICEHo-I-2rc

KN71_001280

MHO_2080

conserved hypothetical lipoprotein

1734

1407

TGGAAAT

(1401–1407) g

PL5_ICEHo-I-1

JRXA01000010.1

JRX01A000001.1

MHO_0120

type-III restriction enzyme

2453

853

TTTTTAAA

(846–853) g

PL5_ICEHo-I-2

JRXA01000004.1

JRXA01000005.1

MHO_1960

hypothetical protein

369

4

AAATAAAG

(4–12) g

SP3615_ICEHo-II

dKGPEAEHF_00485rc d

IGR e

(ddpnA)

methylase DpnIIB

TAATATTA

SP10291_ICEHo-II

HPAMDCMO_00015

MHO_0130

site-specific DNA methyl-transferase

(DEAD/DEAH box helicase family protein)

1200

1152

AAATCTTT

(1145–1152) g

  1. aContigs only shown for strain PL5, due to assembly fragmentation; printed in italics; b MHO genes of PG21 according to acc.-no: FP236530.1; c DR = direct repeat; IR = inverted repeat; d dgene_X = insertion site downstream of gene X; rc = gene on reverse complementary strand; e IGR (= intergenic region) downstream of MHO-homologous gene X (dMHO_X); f downstream DR identical to 3′ end of the gene, protein encoding regions in bold; g DR region in the affected gene (nt x to nt y); h + 1 /+ 2 = direct repeat terminates 1 nt / 2 nt downstream of gene x resulting in downstream DR containing 3′ end of the gene; i no DR, but duplicated region of p75 (nt 1323–1950)