Fig. 2

Experimental validation of the computationally identified structures. a PCR screening for the association of tnp2B with CficCl-61-40 satDNA family arrays. b (1) Fiber-FISH analysis of DNA strands of C. acuminatum. The CficCl-61-40 satDNA family probe (red signal) is associated with arrays (three examples). The bar represents 1 μm. (2) The distribution of CficCl-61-40 satDNA family sequences in the chromosomes of C. album s. str. CficCl-61-40 is labeled with Cy-3 (red signal); chromosomes are stained with DAPI (blue signal). The bar represents 5 μm. c Self-to-self comparisons of the three Oxford Nanopore ultralong reads from the C. acuminatum genome (Additional file 4) displayed as dot plots (YASS program output). Parallel lines indicate tandem repeats (the distance between the diagonals equals the lengths of the motifs). Histograms at the axes indicate the regions with tandem repeats. (1) Read #17 of 49,142 bp; (2) Read #131 of 34,531 bp; (3) Read # 313 of 30,368 bp. The positions of the tnp2B parental fragments are indicated with red arrows, and the associated CficCl-61-40 satDNA family arrays (squares) are indicated with blue arrows