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Fig. 1 | Mobile DNA

Fig. 1

From: Transposons and satellite DNA: on the origin of the major satellite DNA family in the Chenopodium genome

Fig. 1

The tnp2 transposase and the CficCl-61-40 satDNA family. a Schematic representation of contig 22 of the assembled C. acuminatum genome (the first 4000 bp) at different zoom levels. Red squares are the fragments of tnp2. The green line indicates the length of the CficCl-61-40 satDNA array. Blue triangles/squares are conserved motifs of the basic monomer. The green triangle is a similar conserved motif within tnp2 (parental monomer). The red frame indicates the homologous protein sequence of the start monomer and the similar fragment from the other plant species (Cdd: pfam 02992). The positions of PCR primers used for validation of the physical existence of the association of tnp2B with CficCl-61-40 satDNA family arrays are shown with yellow rectangles (see also Fig. 2a). A diagram of the domain organization of the complete CACTA-like TE Jozin is shown at the bottom of a (see also Additional file 3). The 3′ position of the parent for the CficCl-61-40 satDNA array start monomer is shown with an arrow (for further explanation see the text). b Phylogenetic relationships of conserved protein domains of the tnp2 transposase family. Tnp2A in the genomes of the species of the C. album aggregate is highlighted in red. Tnp2B in the genomes of the species of C. album aggregate is highlighted in blue. GenBank accession numbers follow the plant species name. c Phylogenetic relationships of CficCl-61-40 satDNA family monomers and corresponding fragments of tnp2B (the latter are highlighted in blue). A graphical representation of the conservation of CficCl-61-40 satDNA family monomers by sequence logo is shown at the bottom of c (Additional file 2.1)

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