Table 1 Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| HinP1 | New England Biolabs | R0124S |
| Phusion Polymerase | New England Biolabs | M0530S |
| Critical Commercial Assays | ||
| Wizard Genomic DNA Purification Kit | Promega | A1120 |
| Quick Ligation Kit | New England Biolabs | M2200L |
| Qiaquick PCR Cleanup Kit | Qiagen | 28,106 |
| Axygen™ AxyPrep Mag™ PCR Clean-up Kits | Thermo Scientific | 14–223-227 |
| Deposited Data | ||
| Genomic Sequencing Data | This Study | https://www.ncbi.nlm.nih.gov/sra/PRJNA597349 |
| Organisms/Strains | ||
| MP1999 Mucts B::kan. Mu insertion between nts 3,652,046–3,652,051 | (Saha et al. 2013) [28] | ZL530 |
| MG1655 with Mucts:cat at nt 4,203,381 (Ori-Mu) | (Walker et al. 2020) [29] | DMW11 |
| MG1655 with Mucts:cat at nt 263,131 (OPR-Mu) | (Walker et al. 2020) [29] | DMW15 |
| MG1655 with Mucts:cat at nt 3,339,450 (OPL-Mu) | (Walker et al. 2020) [29] | DMW22 |
| MG1655 with Mucts:cat at nt 2,555,144 (TPL-Mu) | (Walker et al. 2020) [29] | DMW24 |
| MG1655 with Mucts:cat at nt 792,226 (TPR-Mu) | (Walker et al. 2020) [29] | DMW33 |
| MG1655 with Mucts:cat at nt 1,657,887 (Ter-Mu) | (Walker et al. 2020) [29] | DMW57 |
| DMW57, Mucts:cat B::kan | This study, DMW57, ZL530 | DMW300 |
| DMW33, Mucts:cat B::kan | This study, DMW33, ZL530 | DMW301 |
| DMW24, Mucts:cat B::kan |
This study, DMW24, ZL530 | DMW302 |
| DMW22, Mucts:cat B::kan |
This study, DMW22, ZL530 | DMW303 |
| DMW15, Mucts:cat B::kan | This study, DMW15, ZL530 | DMW304 |
| DMW11, Mucts:cat B::kan | This study, DMW11 ZL530 | DMW305 |
| Software and Algorithms | ||
| MAPS (Python) | This Study | https://github.com/dmwalker/MuSeq |
| BWA-MEM | Li H., 2013 | https://sourceforge.net/projects/bio-bwa/ |