Fig. 4

MuB is responsible for cis-immunity. The number of insertions near the initial starting location for each Mu prophage was tracked outside both the left and right ends of Mu during EST (early stage transposition; 15 min post-induction of transposition) and LST (late stage transposition; 2 h post-induction). a. The frequency of Mu insertions during EST for all six prophages, both WT and ΔMuB, under four different experimental steps (see text). Pooled experiments are frequency of insertions into that particular Mu location from the other 5 prophages, and indicate that all these particular chromosomal locations are readily transposed into in the absence of Mu. The initial position of Mu is indicated by a yellow line in the center of each plot. b The frequency of Mu insertions per 100 bp as a function of distance outside Mu during EST (top row) and LST (bottom row). The distances reported are combined for both the left and right ends of Mu (see Fig. S4 for individual ends). For bulk DNA, the average number of insertions into a 100 bp region is nearly 25 insertions per 5 million reads during EST, and is indicated by the solid blue line. The shaded blue area is the standard deviation for the number of insertions expected within 100 bp. For OPL-Mu (bottom row), with only one location reporting, the bulk DNA average is around 6 insertions per 100 nucleotides