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Table 4 Step-down PCR thermocycling condition for L1HS amplification

From: Integrated Mobile Element Scanning (ME-Scan) method for identifying multiple types of polymorphic mobile element insertions

95 °C5 min
95 °C1 minRepeat 5 cycles
72 °C1 min
72 °C5 min
95 °C1 minRepeat 5 cycles
68 °C1 min
72 °C5 min
95 °C45 sRepeat 15 cycles
64 °C1 min
72 °C5 min
72 °C15 min
4 °CHold