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Table 4 Step-down PCR thermocycling condition for L1HS amplification

From: Integrated Mobile Element Scanning (ME-Scan) method for identifying multiple types of polymorphic mobile element insertions

95 °C

5 min

95 °C

1 min

Repeat 5 cycles

72 °C

1 min

72 °C

5 min

95 °C

1 min

Repeat 5 cycles

68 °C

1 min

72 °C

5 min

95 °C

45 s

Repeat 15 cycles

64 °C

1 min

72 °C

5 min

72 °C

15 min

4 °C

Hold

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Mobile DNA

ISSN: 1759-8753