Integrated Mobile Element Scanning (ME-Scan) method for identifying multiple types of polymorphic mobile element insertions

Table 2 Oligos and primers used in this study

Description	Sequence (5′- > 3′)
Long adaptor with indexes	CAAGCAGAAGACGGCATACGAGATIndex1GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTIndex2*T
Short adaptor with indexes	*Index2*AGATCGGAAGAGCGTCGTG
1st round AluYb amplification primer	/5Biosg/CAGGCCGGACTGCGGA*C
2nd round AluYb amplification primer	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNAGTGCTGGGATTACAGGCGTG*A
1st round L1HS amplification primer	/5Biosg/GGGAGATATACCTAATGCTAGATGACAC*A
	/5Biosg/GGGAGATATACCTAATGCTAGATGACAC*G
	/5Biosg/GGGAGATATACCTAATGCTAGATGACAA*G
2nd round L1HS amplification primer	TGCACATGTACCCTAAAACTTAGAGTATAA*T
1st round SVA amplification primer	/5Biosg/AGAATCAGGCAGGGAGGTT*G
2nd round SVA amplification primer	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNAGTACMGTCCAGCTTCGGC*T
P7 adaptor amplification primer	CAAGCAGAAGACGGCATACGAGA*T
L1_1 internal primer for validation	GGGAGATATACCTAATGCTAGATGACA
L1_2 internal primer for validation	TGCACATGTACCCTAAAACTTAG
SVA_1 internal primer for validation	AGAATCAGGCAGGGAGGTTG
SVA_2 internal primer for validation	AGTACMGTCCAGCTTCGGCT
/5Biosg/: 5′ Biotin; *: 3′ Phosphorothioate bond; Index1 and Index2: individual specific 6 bp indexes.

ISSN: 1759-8753