Fig. 3
From: A re-evaluation of LINE-1 ORF2 expression in LNCaP prostate cancer cells
ORF2p Immunoprecipitation, immunofluorescence and protein alignment. a Whole cell LNCaP immunoprecipitation (IP) of ORF2p using chA1-L1 antibody. Input, flow through, and IPs were run on a western blot and probed for ORF2 (chA1-L1), ORF2 (MT49), BCLAF1, ORF1, and Tubulin (loading control). b HEK293 cells were transfected with LINE-1, a human CMV ORFeus recoded LINE-1 construct. ORF2p MT5 was used for immunoprecipitation of ORF2p in whole cell lysates overexpressing LINE-1. Vector only lysate (VO), and LINE1 (L1) Input, flow through, and IP (from LINE-1 overexpression lysate) were run on a western blot and probed with ORF2p MT5, ORF2p (chA1-L1), BCLAF1, and tubulin (loading control). c Confocal image of LNCaP cells that were probed for ORF2p using chA1-L1 (red), BCLAF1 (green) and nuclei stained with DAPI (Blue). d MultAlin protein alignment of human BCLAF1 and immunizing ORF2 chA1-L1 peptide