Fig. 2

ORF2p chA1-L1 antibody cross-reacts with BCLAF1. LNCaP cells were treated with Scramble, BCLAF1 (a) or THRAP3 (b) siRNA for 72 h. Whole cell lysates were assayed via western blot and probed with chA1-L1 ORF2p antibody, BCLAF1 or THRAP3, ORF1p, and Tubulin (loading control). Intensity of ORF1 bands in b were normalized to tubulin levels. c) Left: BCLAF1 Tagged overexpression construct with N-terminus 3x flag, V5, and Venus tag (~ 35 kDa). Right: HEK293 cells were transfected with vector only (VO) or tagged BCLAF1 construct. Whole cell lysates were assayed by western blot and probed with ORF2p antibody chA1-L1, and Tubulin (loading control)