Fig. 3
From: LINE-1 ORF2p expression is nearly imperceptible in human cancers
ORF2p mAbs detect endogenous L1Hs. a Epitopes identified by five ORF2p mAbs are indicated along the linear sequence of ORF2p. b IP blockade of ORF2p pulldown can be achieved by pre-incubating ORF2p mAbs with blocking peptides identified in (a). c ORF2p mAb epitopes are highly conserved among both full-length and ORF2-intact L1Hs sequences in the human genome. d Western blot measuring the ability of the MT5 antibody to detect an L1Hs polymorphism at amino acid position 990 reveals that the antibody can detect both alleles. e Epitope %identities among 31 ‘hot’ or highly active L1Hs sequences as reported by Brouha et al. [49]. f Western blot of whole cell lysates (WCL) from several ORF1p negative and ORF1p positive cancer cell lines fails to detect ORF2p with two different ORF2p mAbs. HEK-293TLD cells expressing ORF2-3xFlag are included as a positive control