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Fig. 5 | Mobile DNA

Fig. 5

From: Amplification of miniature inverted-repeat transposable elements and the associated impact on gene regulation and alternative splicing in mulberry (Morus notabilis)

Fig. 5

Effect of the MnM2 element on MnANR expression in transgenic tobacco. a Site-specific PCR analysis using primers designed for sequences flanking MnM2_ scaffold96_203539–203,849 in the following mulberry resources: 1, M. notabilis; 2, M. alba var. Yun3; 3, M. alba var. Lunjiao109; 4, M. alba var. Jialing30; 5, M. alba var. Zhongsang5801; 6, M. alba var. Multicaulis; and 7, M. nigra. M, marker. Bands corresponding to the full site or empty site are indicated. b Schematic diagram of the MnANR, MnANRMF, and MnANRMB constructs. The MnM2 sequence was inserted upstream of the MnANRMF construct and downstream of the MnANRMB construct. c Relative MnANR transcript levels in wild type (WT) and transgenic tobacco seedlings. MnANR, MnANRMF, and MnANRMB correspond to the constructs in b. Transcript levels are presented as fold changes relative to the tobacco actin gene. Error bars represent the standard deviation (n = 3)

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