Fig. 5
From: Targeted gene knockin in zebrafish using the 28S rDNA-specific non-LTR-retrotransposon R2Ol
The transgenic EGFP is expressed in zebrafish. a EGFP expression pattern of the F2 progeny of two R2Ol–EGFP(R) transgenic lines. GRm-1-32 (i), GFm-14-1(ii) and − 14-7 (iii and iv) F1 females were mated with WT. hpf, hours post-fertilization. Dpf, days post-fertilization. Low, middle and high indicate the EGFP expression level. b Heat-shocked F2 progeny of a cross between the R2Ol–GAL4-R (heterozygous) and UAS-GFP lines (homozygous). dpf, days post-fertilization. Each image was taken from an independent mating