Fig. 4
From: Targeted gene knockin in zebrafish using the 28S rDNA-specific non-LTR-retrotransposon R2Ol
PCR analysis of the end of EGFP cassette integrated by r105-R2Ol-EF1-EGFP(R)-r10 injection. a Detection of 5′ end EGFP cassette. b Three founder, GRm-1, GRm-14 and GRf-2 produced R2Ol-EGFP junction fragments (red asterisks). GRm-12 fragment was shorter than expected size (black arrow) and sequencing revealed that it was nonspecific sequence. c Transgenic progeny analysis from three R2Ol-EGFP(R) founders, GRm-1, GRm-14, and GRf-2. Genomic DNA were digested with PvuII