Fig. 2

Germline transmission of R2Ol in zebrafish. a A Founder F0 fish was crossed with WT to obtain F1 progenies. F1 embryos or adults were used for the germline mosaicism analysis. b PCR screening of 3′ junction of R2Ol-positive fish (Red asterisks) using genomic DNA from F1 caudal fins. c 5′ junction sequence of inserted R2Ol. Black box, 28S rDNA sequence; Gray box, R2Ol sequence; Vector, vector sequence; Non-templated, non-template sequence. Truncated or deleted sites are indicated by nucleotide numbers. d Left panel: Southern blot analysis of PCR-positive F1 fish. Right panel: Schematic diagram of three detected R2Ol insertions. P, PvuII site; thick line, probe; thin lines and numbers, signal lengths. Red boxes indicate vector or non-templated sequence