Fig. 6
From: Retrotransposons evolution and impact on lncRNA and protein coding genes in pigs
Sense and antisense expression profiles of pig L1D of L1 s, SINEA of SINEs, and ERV6B of ERVs. a Primer design for reverse transcription (RT) and real-time quantitative PCR (RT-qPCR) detection. The primer for sense and antisense RT are indicated by red and green arrowheads, respectively, and the primers of ORF1-F/R, ORF2-F/R, 5′UTR-F/R, pol-F/R, gag-F/R, env-F/R, LTR-F/R, SINE-F/R (black arrowheads), are used for RT-qPCR to detect the expression of 5′UTR, ORF1, and ORF2 of L1, LTR, gag, pol, and env of ERV6 and SINE, respectively. b Sense expression of ORF1 and ORF2, and antisense expression of 5′UTR of L1D in tissues and cells. c Sense and antisense expression of SINEA in tissues and cells. d Sense expression of gag, pol, and env of ERV6, and antisense expression of LTR of ERV6 in tissues and cells