Fig. 6
From: Identification of charged amino acids required for nuclear localization of human L1 ORF1 protein
Transient overexpression of both KPNA2 and KPNB1 significantly increases L1 retrotransposition in HeLa cells. HeLa cells were transiently co-transfected with the L1Neo expression plasmid and plasmids expressing KPNA2 and/or KPNB1. All data normalized for toxicity that was determined by co-transfection of these expression plasmids with a plasmid expressing neomycin resistance gene (Additional file 5: Figure S5). Representative flasks are shown and the average number of colonies +/− standard deviation are indicated for each transfection condition. Error bars show standard deviation determined using data from three independent experiments (*, p < .05; ****, p < .0001)