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Fig. 5 | Mobile DNA

Fig. 5

From: Identification of charged amino acids required for nuclear localization of human L1 ORF1 protein

Fig. 5

Interaction between ORF1p and KPNA2 is disrupted by either mutation of RRM and CTD lysines or RNase treatment. a HeLa cells were transiently co-transfected with plasmids expressing FLAG-tagged or untagged KPNA2 proteins and plasmids expressing wildtype or mutant human ORF1p. Co-Immunoprecipitation was performed with Anti-FLAG beads. Western blot analysis was performed using Anti-FLAG antibodies, hORF1 antibodies, and Actin loading control antibodies. Red boxes indicate FLAG-tagged proteins. Control indicates transfection with an empty plasmid. b Quantification of co-immunoprecipitation results shown as percentage of immunoprecipitated 14 K:A ORF1p as compared to Wildtype ORF1p. Error bars show standard deviation determined using data from three independent experiments (*, p < .05). c HeLa cells were transiently co-transfected with plasmids expressing FLAG-tagged KPNA2 proteins and plasmids expressing wildtype human ORF1p. Co-Immunoprecipitation was performed with Anti-FLAG beads. Western blot analysis was performed using Anti-FLAG antibodies, hORF1 antibodies, and Actin loading control antibodies. Red boxes indicate FLAG-tagged proteins. Control indicates transfection with an empty plasmid. d Quantification of co-immunoprecipitation results shown as percentage of immunoprecipitated ORF1p with RNase as compared to immunoprecipitated ORF1p without RNase. Error bars show standard deviation determined using data from three independent experiments (**, p < .01)

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