Fig. 5From: Identification of charged amino acids required for nuclear localization of human L1 ORF1 proteinInteraction between ORF1p and KPNA2 is disrupted by either mutation of RRM and CTD lysines or RNase treatment. a HeLa cells were transiently co-transfected with plasmids expressing FLAG-tagged or untagged KPNA2 proteins and plasmids expressing wildtype or mutant human ORF1p. Co-Immunoprecipitation was performed with Anti-FLAG beads. Western blot analysis was performed using Anti-FLAG antibodies, hORF1 antibodies, and Actin loading control antibodies. Red boxes indicate FLAG-tagged proteins. Control indicates transfection with an empty plasmid. b Quantification of co-immunoprecipitation results shown as percentage of immunoprecipitated 14 K:A ORF1p as compared to Wildtype ORF1p. Error bars show standard deviation determined using data from three independent experiments (*, p < .05). c HeLa cells were transiently co-transfected with plasmids expressing FLAG-tagged KPNA2 proteins and plasmids expressing wildtype human ORF1p. Co-Immunoprecipitation was performed with Anti-FLAG beads. Western blot analysis was performed using Anti-FLAG antibodies, hORF1 antibodies, and Actin loading control antibodies. Red boxes indicate FLAG-tagged proteins. Control indicates transfection with an empty plasmid. d Quantification of co-immunoprecipitation results shown as percentage of immunoprecipitated ORF1p with RNase as compared to immunoprecipitated ORF1p without RNase. Error bars show standard deviation determined using data from three independent experiments (**, p < .01)Back to article page