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Fig. 4 | Mobile DNA

Fig. 4

From: The impact of transposable element activity on therapeutically relevant human stem cells

Fig. 4

Use of class II transposons as gene vectors. a Autonomous DNA transposons consist of a transposase-coding gene that is flanked by inverted terminal repeats (ITR; black arrows flanked by white arrows). b Bi-component transposon vector system for delivering plasmid-encoded transgenes. One component consists of a plasmid containing a gene of interest (GOI) flanked by transposon ITRs. The second component is a transposase expression plasmid. Black arrow, promoter driving expression of transposase gene. c The transposon carrying a GOI is excised from the donor plasmid and integrated at a chromosomal site by the transposase

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