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Table 1 Putative stability determinants identified in the three native plasmids of P. syringae pv. savastanoi NCPPB 3335

From: The toxic guardians — multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids

Plasmid and determinanta

Locus Tagb

Deduced product (InterPro family or signature matches)

pPsv48A

 SD1

PSPSV_A0016

Putative partition protein A (IPR027417, IPR025669)

 

PSPSV_A0015

Ribbon-helix-helix protein, CopG family (none predicted)

 TA1

PSPSV_A0020

Putative addiction module antitoxin, RelB/DinJ family protein (IPR007337)

 

PSPSV_A0019

Putative toxin of the YafQ-DinJ toxin-antitoxin system (IPR004386), addiction module toxin, RelE/StbE family (IPR007712)

 TA2

PSPSV_A0032

Predicted transcriptional regulator, ribbon-helix-helix protein, CopG (IPR010985)

 

PSPSV_A0031

Putative plasmid stabilization system protein; RelE/ParE toxin family (IPR007712)

 SD2c

PSPSV_A0042

StbA, putative stability/partitioning determinant, resolvase (IPR036162)

 

PSPSV_A0041

MvaT-like transcriptional regulator (IPR035616)

 TA3

PSPSV_A0043d

StbC, Arc-type ribbon-helix-helix protein, putative antitoxin (IPR013321)

 

PSPSV_A0044d

StbB, putative ribonuclease of the VapC family (IPR022907)

PSPSV_A0045d

StbA, resolvase (IPR036162)

 TA4

PSPSV_A0051

Putative RelB/DinJ family addiction module antitoxin (none predicted)

 

PSPSV_A0050

Putative RelE/StbE family addiction module toxin (IPR007712)

 SD3

PSPSV_A0067

Putative transcriptional regulator; CopG/Arc/MetJ DNA-binding domain-containing protein, possibly responsible for the regulation of plasmid copy number (IPR002145)

pPsv48B

 TA5

PSPSV_B0012

Hypothetical protein, putative plasmid maintenance component (IPR021558, DUF3018)

 

PSPSV_B0011

Putative Mazf-like toxin, ccdB family (IPR003477)

 SD4

PSPSV_B0013

ParA/YafB type stability/partitioning protein, cobyrinic acid ac-diamide synthase (IPR027417)

 

PSPSV_B0014

Stability/partitioning protein (none predicted)

 SD5

PSPSV_B0038

IncN plasmid killer protein (IPR009989)

 SD6

PSPSV_B0042

Putative stability/partitioning determinant (IPR027417)

 

PSPSV_B0043

Hypothetical protein (none predicted)

pPsv48C

 TA6

PSPSV_C0003

Putative RelE/StbE family antitoxin, stability determinant (none predicted)

 

PSPSV_C0004

Putative RelE/StbE family toxin, stability determinant (IPR007712)

 TA7

PSPSV_C0008

Putative CopG family transcriptional regulator (IPR010985; IPR013321)

 

PSPSV_C0007

Putative addiction module toxin, plasmid stabilization protein (IPR007712)

 SD7e

PSPSV_C0017

Putative ParA family protein (IPR027417)

 

not annotated

Hypothetical protein (none predicted)

 TA8

PSPSV_C0050

Putative antitoxin (none predicted)

 

PSPSV_C0051

Putative addiction module toxin, RelE/StbE family (IPR007712)

  1. aTA toxin-antitoxin system, SD generic stability determinant
  2. bThe listing order indicates direction of transcription
  3. cGene PSPSV_A0042 shows 90% nt identity to gene PSPSV_A0045 in determinant TA3
  4. dGenes PSPSV_A0043/44/45 are 100% identical to genes PSPSV_A0007/8/9, respectively
  5. eSD7 was cloned containing an unannotated CDS 3′ of PSPSV_C0017 (fragment containing positions 9861–11,121 of FR820587), which could be part of a par operon