Fig. 5

Types of deletions of pPsv48C::sacB as influenced by functional toxin-antitoxin systems. a Left: Map of pPsv48C::sacB; TA6, TA7 and TA8, toxin-antitoxin systems; C43, locus PSPSV_C0043; inverted triangle, Km^R-sacB cassette cloned 0.1 kb 3′ of the IS801 isoform. Lines under the map indicate the minimum (black line) and maximum (dotted line) extent of DNA transposed by IS801 on each group of suc^R plasmids. Right: Presence (+) or absence (−) of specific amplicons for each of the genes shown, or of resistance (+) and sensitivity (−) to kanamycin. Last two columns indicate the percentage of suc^R colonies containing each plasmid group in UPN1007 containing the empty vector pRK415 (310 colonies analysed) or pRK3C, leading to functional inactivation of the TA systems (323 colonies analysed). Gels showing typical patterns of multiplex PCR amplifications (panel b) and uncut plasmids (panel c) of example clones from each plasmid group. M, molecular weight markers, in kb; clp, chromosomal DNA and linearized plasmids. Lanes: (1) P. syringae pv. savastanoi NCPPB 3335; (2) Psv48ΔAB, containing only pPsv48C; and (3) UPN864, containing only pPsv48C::sacB