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Fig. 3 | Mobile DNA

Fig. 3

From: The toxic guardians — multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids

Fig. 3

Recombination between two directly repeated copies of MITEPsy2 causes a deletion on pPsv48C. a Partial map of pPsv48C::Tn5-GDYN1 (pC::Tn5) showing the relative positions of its only copy of the IS801 isoform, its two replication initiation protein genes (repJ and repA), and toxin-antitoxin system 8 (TA8). Green block arrows, MITEPsy2; inverted black triangle, Tn5-GDYN1 (Tn). pCΔ1 is pPsv48CΔ1, containing an 8.3 kb deletion resulting from MITEPsy2 recombination. b Electrophoresed uncut plasmid preparations from: (1) P. syringae pv. savastanoi NCPPB 3335; (2) Psv48ΔAB; (3) UPN827, and (4) UPN864. pA, pPsv48A; pB; pPsv48B; pC, pPsv48C; pCΔ1, pPsv48CΔ1; clp, chromosomal DNA and linearized plasmids. Lanes were loaded with equivalent amounts of cell lysates; results are representative of at least 20 independent plasmid preparations

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