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Table 4 Ligation master mix

From: Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome

Ligation master mix

Volume (Ī¼L)

1x

8x

10ā€‰mM ATP

2.5

20

10x T4 Ligase buffer

0.5

4.0

T4 Ligase (400ā€‰U/Ī¼L)

0.2

1.6

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Mobile DNA

ISSN: 1759-8753