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Table 3 Digestion master mix

From: Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome

Digestion master mix

Volume (Ī¼L)

1x

4x

Molecular grade H2O

2.25

9.0

10x Restriction enzyme buffer

2.5

10

Restriction enzyme

1.0

4.0

RNase cocktail enzyme mix

0.25

1.0