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Table 2 Vectorette PCR thermal cycler program

From: Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome

95 °C

5 min

95 °C

1 min

5 cycles

72 °C

1 min

72 °C

5 min

95 °C

1 min

5 cycles

68 °C

1 min

72 °C

5 min

95 °C

45 s

15 cycles

64 °C

1 min

72 °C

5 min

95 °C

45 s

15 cycles

60 °C

1 min

72 °C

5 min

72 °C

15 min

16 °C

Hold

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Mobile DNA

ISSN: 1759-8753