Table 1 Vectorette oligo and primer sequences
Enzyme Vectorette Oligo Sequences (5′ to 3′) | |
AseI plus | TAGAAGGAGAGGACGCTGTCTGTCGAAGGTAAGGAACGGACGAGAGAAGGGAGAG |
BspHI plus | CATGGAAGGAGAGGACGCTGTCTGTCGAAGGTAAGGAACGGACGAGAGAAGGGAGAG |
BstYI plus | GATCGAAGGAGAGGACGCTGTCTGTCGAAGGTAAGGAACGGACGAGAGAAGGGAGAG |
HindIII plus | AGCTGAAGGAGAGGACGCTGTCTGTCGAAGGTAAGGAACGGACGAGAGAAGGGAGAG |
NcoI plus | CATGGAAGGAGAGGACGCTGTCTGTCGAAGGTAAGGAACGGACGAGAGAAGGGAGAG |
PstI minus | CTCTCCCTTCTCGGATCTTAACCGTTCGTACGAGAATCGCTGTCCTCTCCTTCTGCA |
Common Vectorette Oligo Sequences (5′ to 3′) | |
Vectorette minus | CTCTCCCTTCTCGGATCTTAACCGTTCGTACGAGAATCGCTGTCCTCTCCTTC |
Vectorette plus | GAAGGAGAGGACGCTGTCTGTCGAAGGTAAGGAACGGACGAGAGAAGGGAGAG |
Primer Sequences (5′ to 3′) | |
L1 Primer | AGATATACCTAATGCTAGATGACACA |
Vectorette Primer | CTCTCCCTTCTCGGATCTTAA |