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Table 3 Oligonucleotide primers used in the study

From: Features of a novel protein, rusticalin, from the ascidian Styela rustica reveal ancestral horizontal gene transfer event

 

Primer

Sequence 5′-3′

1

Sr_P26_3’_F1

ggnaaywsntayathmng

2

Sr_P26_3’_F2

tasttayattcgttgt

3

Sr_P26_5’_R1

cattgtgccaagttcccgag

4

Sr_P26_5’_R2

ccgagcaatggttgctgttta

  1. h = a,c,t; y = c,t; s = c,g; n = a,g,c,t; m = a,c; w = a,t. Bold letters with underscore – overlaying parts of nested primers