Table 1 Results of iterative search strategy used to identify rusticalin-like proteins
Species | Identification method | Sequence ID | Protein existence | Preprotein aa | Predicted cellular location | Predicted signal peptide | Putative mature protein | ||
|---|---|---|---|---|---|---|---|---|---|
Molecular mass Da | pI | DE/RK | |||||||
Botryllus schlosseri | TBLASTN | gb|JG375642.1| gb|JG376766.1| | transcript | 271 | extracellular | 1–19 | 26,843 | 4.30 | 27/10 |
Ciona intestinalis | HHblits | XP_002128942.1 | predicted ORF | 271 | extracellular | 1–16 | 28,004 | 8.36 | 24/29 |
Ciona intestinalis | HHblits | XP_002122335.1 | predicted ORF | 267 | extracellular | 1–17 | 26,759 | 4.32 | 27/12 |
Ciona intestinalis | TBLASTN | GBKV01021559.1 | transcript | 271 | extracellular | 1–16 | 28,184 | 7.84 | 27/29 |
Ciona savignyi | TBLASTN | GGEI01017847.1 | transcript | 271 | extracellular | 1–15 | 27,988 | 8.06 | 26/29 |
Diplosoma listerianum | TBLASTN | gb|CN499160.1| gb|CN499008.1| | transcript | 267 | extracellular | 1–17 | 26,889 | 4.39 | 28/12 |
Oikopleura dioica | TBLASTN | emb|FP744447.1| | transcript | 263 | extracellular | 1–14 | 27,192 | 5.67 | 29/24 |
Oikopleura dioica | TBLASTN | GCJN01018270.1 | transcript | 260 | extracellular | 1–16 | 26,642 | 5,96 | 28/25 |
Asymmetron lucayanum | TBLASTN | GESY01031038.1 | transcript | 264 | extracellular | 1–16 | 26,489 | 5.43 | 26/20 |
Branchiostoma floridae | HHblits | XP_002588042.1 dbj|BW723234.1| | predicted ORF transcript | 268 | extracellular | 1–15 | 27,117 | 5.10 | 28/20 |
Branchiostoma floridae | TBLASTN | GETA01020221.1 | transcript | 270 | extracellular | 1–16 | 27,235 | 5.01 | 30/21 |
Alveopora japonica | TBLASTN | GGJR01103857.1 | transcript | 269 | extracellular | 1–18 | 27,585 | 4.29 | 29/12 |
Alveopora japonica | TBLASTN | GGJR01116144.1 | transcript | 256 | extracellular | 1–18 | 25,764 | 4.23 | 29/12 |
Trichoplax adhaerens | HHblits | XP_002117795.1 gb|GR416119.1| | predicted ORF transcript | 248 | extracellular | 1–18 | 24,575 | 7.51 | 13/14 |