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Table 1 Results of iterative search strategy used to identify rusticalin-like proteins

From: Features of a novel protein, rusticalin, from the ascidian Styela rustica reveal ancestral horizontal gene transfer event

Species Identification method Sequence ID Protein existence Preprotein aa Predicted cellular location Predicted signal peptide Putative mature protein
Molecular mass Da pI DE/RK
Botryllus schlosseri TBLASTN gb|JG375642.1| gb|JG376766.1| transcript 271 extracellular 1–19 26,843 4.30 27/10
Ciona intestinalis HHblits XP_002128942.1 predicted ORF 271 extracellular 1–16 28,004 8.36 24/29
Ciona intestinalis HHblits XP_002122335.1 predicted ORF 267 extracellular 1–17 26,759 4.32 27/12
Ciona intestinalis TBLASTN GBKV01021559.1 transcript 271 extracellular 1–16 28,184 7.84 27/29
Ciona savignyi TBLASTN GGEI01017847.1 transcript 271 extracellular 1–15 27,988 8.06 26/29
Diplosoma listerianum TBLASTN gb|CN499160.1| gb|CN499008.1| transcript 267 extracellular 1–17 26,889 4.39 28/12
Oikopleura dioica TBLASTN emb|FP744447.1| transcript 263 extracellular 1–14 27,192 5.67 29/24
Oikopleura dioica TBLASTN GCJN01018270.1 transcript 260 extracellular 1–16 26,642 5,96 28/25
Asymmetron lucayanum TBLASTN GESY01031038.1 transcript 264 extracellular 1–16 26,489 5.43 26/20
Branchiostoma floridae HHblits XP_002588042.1 dbj|BW723234.1| predicted ORF transcript 268 extracellular 1–15 27,117 5.10 28/20
Branchiostoma floridae TBLASTN GETA01020221.1 transcript 270 extracellular 1–16 27,235 5.01 30/21
Alveopora japonica TBLASTN GGJR01103857.1 transcript 269 extracellular 1–18 27,585 4.29 29/12
Alveopora japonica TBLASTN GGJR01116144.1 transcript 256 extracellular 1–18 25,764 4.23 29/12
Trichoplax adhaerens HHblits XP_002117795.1 gb|GR416119.1| predicted ORF transcript 248 extracellular 1–18 24,575 7.51 13/14