Fig. 6From: High-performance gene expression and knockout tools using sleeping beauty transposon systemDepletion of BRD7 leads to spindle misorientation. a Cells were transfected with control or BRD7 sgRNAs and stained with anti-β-tubulin antibody (red) and DAPI (blue) and the image series of mitotic cells were shown. The position of the Z stage of the mitotic spindle is indicated in μm, and stack refers to the projected image. b Scheme for analysis of various parameters of the mitotic spindle: spindle angle (α) and spindle length (μm). (D) XY and XZ projections of confocal Z stacks taken of metaphase cells stained for β-tubulin (red) and DNA (blue). c and e Experiments were performed as in A, and the spindle length, the spindle angle between the two spindle poles were measured as described in B. f and g Immunofluorescence/phase-contrast images and cell diameter of control and BRD7-depleted metaphase HeLa cells stained with anti-β-tubulin antibody (red) and DNA (blue). n = 30 cells per group. Values are mean ± SD. *, p < 0.05, **, p < 0.01. NS, not significant. Scale bars, 2 μmBack to article page