Fig. 5

BRD7 is important for oriented cell division. a Immunoblots for BRD7 and target gene expression (ERα and RAD51) in control and BRD7-depleted HeLa cells (n = 3). b Wild-type and BRD7-depleted HeLa cells were incubated with nocodazole (100 ng/ml) for 16 h, harvested and analyzed using flow cytometry. The percentage of cells positive for pH 3 is indicated (n = 3). c Time-lapse images showing prolonged prometaphase and telophase and misoriented cell division (uneven timing of daughter-cell adhesion to the substratum) in BRD7-depleted HeLa-H2B cells, compared with control. Dashed lines indicate misoriented cell divisions. (Scale bars, 2 μm). d Quantification of mitotic cells showed in C (n = 15 mitotic cells per group) and results represent mean ± SD. Error bars indicate SD. *, p < 0.05. e Quantification of normal and misoriented cell divisions in cells treated as in C (n = 15 mitotic cells per group). Values are mean ± SD. *, p < 0.05. NS, not significant