Fig. 1
From: High-performance gene expression and knockout tools using sleeping beauty transposon system
Overview of the Sleeping Beauty (SB) transposon system. a The Gateway cloning of pSB vectors. cDNA is cloned into an Entry vector between the attL1 and attL2 sites. In the presence of the LR clonase, recombination occurs between attL1-attR1 and attL2-attR2 to transfer the insert from the Entry vector into the Destination vector of choice. All Entry vectors contain the kanamycin resistance gene whereas all Destination vectors carry the ampicillin resistance gene. b sgRNA can be expressed in either pSB-CRISPR-Puro or pSB-CRISPR-Blast vector for protein depletion. c The workflow for using the Sleeping Beauty transposon system. Puro, puromycin; Blast, blasticidin