Fig. 4

Results of putative somatic Alu locus-specific PCR. Marker – 100 bp DNA ladder (Evrogen); cortex – results of semi-nested PCR with “normalization 2” library; myocardium - results of semi-nested PCR with control library (myocardium from the same donor); N1-N12 somatic insertions with UMI count more than 1. Blue arrows indicate PCR products of expected size. For N4 insertion amplification product of expected size was observed in both “normalization 2” and control myocardium PCR reactions. For N8 insertion amplification product of expected size was not detected neither in “normalization 2” nor in control myocardium PCR reaction. An example (N9 insertion) of capillary sequencing result is shown at the bottom