Fig. 2
From: An advanced enrichment method for rare somatic retroelement insertions sequencing
qPCR analysis of selected insertions and spike-in controls. Each dot indicates Ct values for each Alu flanking sequence in “unnormalized”, “normalization 1” and “normalization 2” libraries. Orange dots – fixed insertions (FI) present in each cell, blue dots – somatic insertions (SI) present in more than one cell, green dots (DR) – spike-in controls containing artificial sequences from Danio rerio. The difference in Ct between abundant fixed insertions and rare spike-in insertions changed from 25 cycles for “unnormalized” to 10 cycles for “normalization 2” libraries