Insertion sequence elements-mediated structural variations in bacterial genomes

Table 1 IS-mediated structural variation rates across bacterial genomes

Bacteria strain	Genome size	Number of	Generations	Insertions		Deletions		Reference
	[Mbps]	MA lines	per MA line	IS-related	Rate [ ×10⁻⁴]	IS-related	Rate [ ×10⁻⁵]
B. cenocepacia HI2424	7.70	50	5500	57	2.10			This study
V. cholerae 2740-80 MMR mut	4.09	48	1254	1	0.02			This study
M. smegmatis MC2 155	6.99	49	4900	106	4.40	202	84.0	This study
D. radiodurans BAA-816	3.8	43	5961	640	25.0			[23]
D. radiodurans R1 (ATCC13949) mutL-	3.8	19	993	9	4.80			[23]
E. coli K12 MG1655	4.64	520	4186	758	3.50	98	4.50	[21]
E. coli REL4536 (Aerobic)	4.63	24	4500	58	5.40	78	72.0	This study
E. coli REL4536 (Anaerobic)	4.63	24	3456	166	20.0	78	94.0	This study
E. coli ED1a	5.21	50	6114	7	0.23	11	3.60	This study
E. coli IAI1	4.7	49	6342	-	-	2	0.64	This study

In previous studies, the IS insertion rate was reported to be approximately 2.5×10⁻³ insertions per genome per generation in the wild type of D. radiodurans BAA-816, which is much higher than the rate of 4.8×10⁻⁴ IS insertions per genome per generation observed in D. radiodurans R1 (ATCC13949) mismatch repair (MMR)-deficient strain [23], and the rate of 3.5×10⁻⁴ IS insertions per genome per generation reported in E. coli K12 MG1655 [21]. Our results indicate that the insertion and recombination rates of IS elements vary between different bacterial genomes and even among strains of the same species

ISSN: 1759-8753