Fig. 4
From: F-CphI represents a new homing endonuclease family using the Endo VII catalytic motif
Activity comparison of F-CphI wild type and mutants. F-CphI wild type (400 nM) and mutants (400 nM and 3000 nM for N118A; 400 nM and 4000 nM for others) were incubated with 5 nM closed-circular plasmid DNA (C) at 25 °C for 1 h. For N118A, 4000 nM cannot be used since it bound to the DNA substrate non-specifically and showed a smear in the gel (data not shown). Reaction products were separated on a 0.8% agarose gel. Positions of linear (L) and open circular (O) products are shown. DNA substrate without protein added was run in the last lane