Fig. 3
From: F-CphI represents a new homing endonuclease family using the Endo VII catalytic motif
Optimization of conditions for DNA cleavage by F-CphI. Purified F-CphI (1600Â nM) was used to digest plasmid DNA (5Â nM) in different conditions for 30Â min. The graphs show F-CphI endonuclease activity at different temperatures a, pH b, Mg2+ concentrations c, monovalent cation concentrations d and divalent cation concentrations e F-CphI endonuclease activity was calculated by expressing intensities of the linear products relative to the total plasmid DNA substrates. The highest activity for each variable was assigned a value of 100%. Error bars indicate the s.d. from two b, d and e or three a and c experiments