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Fig. 1 | Mobile DNA

Fig. 1

From: Length variations within the Merle retrotransposon of canine PMEL: correlating genotype with phenotype

Fig. 1

Evaluation of an assay for determining the length of the Merle SINE oligo(dT). (a) Sequence of the PCR product is shown, with primer sequences in bold. The retrotransposon is underlined, and the oligo(dT) is in blue. Non-oligo(dT) sequences total 234 bp. The wild-type splice site is in purple, while the alternative splice site is in red. (b) A chromatogram from fragment analysis depicts amplicon size in base pairs (x-axis) and signal intensity in relative fluorescent units (RFU) (y-axis). For determination of oligo(dT) length, 234 is subtracted from the size of the amplicon peak with the highest RFU (highlighted), rounded to the nearest whole number. (c) Standard deviation of amplicon size is given for technical replicates from one dog representing each of four phenotypes. (d) Average signal intensity from the technical replicates is plotted against average amplicon size

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